Rapid communication: mapping the pig VCAM1 locus to chromosome 4 using a double-stranded conformation polymorphism marker (VCAM1-2).
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منابع مشابه
Rapid communication: mapping the pig VCAM1 locus to chromosome 4 using a double-stranded conformation polymorphism marker (VCAM1-2).
Source and Description of Primers. We previously identified a SacI polymorphism by using a pig VCAM1 cDNA probe on Southern blots (VCAM1-1; Helm et al., 1994). This polymorphism was not informative enough to map VCAM1. To develop PCR-based genotyping, we sequenced the 3¢ untranslated region of pig VCAM1. Subsequently, a pig VCAM1 cDNA was deposited in Genbank; our data agree completely with tha...
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Probe Source and Description. A randomly selected 1.3-kb cDNA clone (S28) was isolated from a porcine spleen library (CLONTECH j. Amplification and hybridization analysis (previously described; Tuggle and Schmitz, 1994) indicated S28 was specifically expressed in spleen. A 504-bp BgZII-BarnHI subclone of S28 was sequenced and identified as a porcine vascular cellular adhesion molecule ( VCAM) c...
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Name of Gene Marker. MBL2, Mannose-Binding Lectin 2 (Mannose-Binding Protein, Serum; MBP1). Source and Description of Primers. Primers (set A) were designed based on human (GenBank accession number X15422) and cattle (GenBank accession number D73408) MBL2 sequences available in the GenBank database. Using porcine genomic DNA as a template in the PCR, these primers amplified an 831-bp fragment. ...
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Source and Description of Primers. The initial primers for the PCR were designed based on human DNA sequence (accession no. X92412; Kolmerer et al., 1996). The position of the forward and reverse primers corresponded to exon 3 and exon 5, respectively. These primers are expected to amplify a fragment of 1.93 kb from human DNA. A PCR fragment of 2.1 kb was amplified from porcine genomic DNA. Par...
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ژورنال
عنوان ژورنال: Journal of Animal Science
سال: 1997
ISSN: 0021-8812
DOI: 10.2527/1997.7582286x